Articles and News for Jul08
  1. 1H NMR metabolite fingerprinting and metabolomic analysis of perchloric acid extracts from plant tissues

  2. 3DNA: a versatile, integrated software system for the analysis, rebuilding and visualization of three-dimensional nucleic-acid structures

  3. A computational method of prediction of the end-diastolic pressure?volume relationship by single beat

  4. A continuous process to extract plasmid DNA based on alkaline lysis

  5. A fluorescence-based double retrograde tracer strategy for charting central neuronal connections

  6. A high-throughput fluorescence-based glycosyltransferase screen and its application in directed evolution

  7. A light microscope-based double retrograde tracer strategy to chart central neuronal connections

  8. A membrane capture assay for lipid kinase activity

  9. A method for quantifying aggression in male Drosophila melanogaster

  10. A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies

  11. A protocol for a lung neovascularization model in rodents

  12. A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond

  13. A protocol for culturing Drosophila melanogaster stage 9 egg chambers for live imaging

  14. A protocol for designing siRNAs with high functionality and specificity

  15. A protocol for determination of anticardiolipin antibodies by ELISA

  16. A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice

  17. A protocol for isolation and visualization of yeast nuclei by scanning electron microscopy (SEM)

  18. A protocol for phenotypic detection and characterization of vascular cells of different origins in a lung neovascularization model in rodents

  19. A protocol for the in vitro selection of specific oligonucleotide probes for high-resolution DNA typing

  20. A rapid micro chromatin immunoprecipitation assay (ChIP)

  21. A reproducible and well-tolerated method for 2/3 partial hepatectomy in mice

  22. A universal real-time PCR assay for the quantification of group-M HIV-1 proviral load

  23. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances

  24. Agrobacterium-mediated transformation of Brassica napus and Brassica oleracea

  25. Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed

  26. An ESI-MS method for characterization of native and modified oligonucleotides used for RNA interference and other biological applications

  27. An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in Arabidopsis plants

  28. An expedient, facile and simple one-pot synthesis of 2-methylenealkanoates and alkanenitriles from the Baylis?Hillman bromides in aqueous media

  29. An inducible mouse model of colon carcinogenesis for the analysis of sporadic and inflammation-driven tumor progression

  30. An optimized method for measurement of gamma-H2AX in blood mononuclear and cultured cells

  31. An optimized procedure for whole-mount in situ hybridization on mouse embryos and embryoid bodies

  32. Analysis of epigenetic modifications of chromatin at specific gene loci by native chromatin immunoprecipitation of nucleosomes isolated using hydroxyapatite chromatography

  33. Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells

  34. Analysis of mutational spectra by denaturing capillary electrophoresis

  35. Analyzing grooming microstructure in neurobehavioral experiments

  36. Application of cationic conjugated polymers in microarrays using label-free DNA targets

  37. Array-MAPH: a methodology for the detection of locus copy-number changes in complex genomes

  38. Artificial restriction DNA cutter for site-selective scission of double-stranded DNA with tunable scission site and specificity

  39. Assessing the delivery efficacy and internalization route of cell-penetrating peptides

  40. Assessment of colon sensitivity by luminal distension in mice

  41. Asymmetric aldol reaction using boron enolates

  42. Atomic force microscopy and chemical force microscopy of microbial cells

  43. Atomic force microscopy and spectroscopy of native membrane proteins

  44. Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7

  45. Bicelle samples for solid-state NMR of membrane proteins

  46. Bioluminescence imaging of hollow fibers in living animals: its application in monitoring molecular pathways

  47. Cell-PLoc: a package of Web servers for predicting subcellular localization of proteins in various organisms

  48. Cell cycle synchronization of animal cells and nuclei by centrifugal elutriation

  49. Changing medium and passaging cell lines

  50. Characterizing peptides in individual mammalian cells using mass spectrometry

  51. Colocalization of fluorescent markers in confocal microscope images of plant cells

  52. Competitive PCR?ELISA protocols for the quantitative and the standardized detection of viral genomes

  53. Competitive PCR for precise nucleic acid quantification

  54. Creating transgenic Drosophila by microinjecting the site-specific ?C31 integrase mRNA and a transgene-containing donor plasmid

  55. Cryosectioning and immunolabeling

  56. Cu-catalyzed Goldberg and Ullmann reactions of aryl halides using chelating N- and O-based ligands

  57. Culture of Drosophila S2 cells and their use for RNAi-mediated loss-of-function studies and immunofluorescence microscopy

  58. Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro

  59. DMS footprinting of structured RNAs and RNA?protein complexes

  60. DNA methylation analysis by pyrosequencing

  61. Derivation of human embryonic stem cells from single blastomeres

  62. Design and implementation of high-throughput RNAi screens in cultured Drosophila cells

  63. Designing candidate gene and genome-wide case?control association studies

  64. Detecting antigens by quantitative immuno-PCR

  65. Detecting ligands and dissecting nuclear receptor-signaling pathways using recombinant strains of the yeast Saccharomyces cerevisiae

  66. Detection of 2-hydroxyethidium in cellular systems: a unique marker product of superoxide and hydroethidine

  67. Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyramide signal amplification

  68. Detection of protein?protein interactions using a simple survival protein-fragment complementation assay based on the enzyme dihydrofolate reductase

  69. Detergent resistance as a tool in membrane research

  70. Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers

  71. Digital karyotyping

  72. Direct detection of N?Hct?N hydrogen bonds in biomolecules by NMR spectroscopy

  73. Direct detection of N?Hct?O=C hydrogen bonds in biomolecules by NMR spectroscopy

  74. Direct detection of small RNAs using splinted ligation

  75. Displacement of protein-bound aptamers with small molecules screened by fluorescence polarization

  76. Dissecting RNA folding by nucleotide analog interference mapping (NAIM)

  77. Electrophoretic mobility shift assay (EMSA) for detecting protein?nucleic acid interactions

  78. Enzymatic ligation assisted by nucleases: simultaneous ligation and digestion promote the ordered assembly of DNA

  79. Estimation of nuclear DNA content in plants using flow cytometry

  80. Explanatory signal interpretation and metabolite identification strategies for nominal mass FIE-MS metabolite fingerprints

  81. Fabrication of carbohydrate chips and their use to probe protein?carbohydrate interactions

  82. Facile synthesis of Ag nanocubes and Au nanocages

  83. Fluorescence and chromogenic in situ hybridization to detect genetic aberrations in formalin-fixed paraffin embedded material, including tissue microarrays

  84. Fluorescence detection of protein clusters in individual cells and tissue sections by using toponome imaging system: sample preparation and measuring procedures

  85. Fluorescent Arabidopsis tetrads: a visual assay for quickly developing large crossover and crossover interference data sets

  86. Fluorescent high-throughput screening of chemical inducers of neuronal differentiation in skeletal muscle cells

  87. Frontal affinity chromatography: sugar?protein interactions

  88. Frontal affinity chromatography?mass spectrometry

  89. GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

  90. Generation of human-induced pluripotent stem cells

  91. Generation of magnetic nonviral gene transfer agents and magnetofection in vitro

  92. Genetic knockouts and knockins in human somatic cells

  93. High-efficiency transformation by biolistics of soybean, common bean and cotton transgenic plants

  94. High-resolution in situ hybridization to whole-mount zebrafish embryos

  95. Identification of enzymes and activity from two-dimensional gel electrophoresis

  96. Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays

  97. Image processing for electron microscopy single-particle analysis using XMIPP

  98. In vitro functional imaging in brain slices using fast voltage-sensitive dye imaging combined with whole-cell patch recording

  99. Inhibitor fingerprinting of metalloproteases using microplate and microarray platforms: an enabling technology in Catalomics

  100. Integration of biological networks and gene expression data using Cytoscape

  101. Ion mobility?mass spectrometry analysis of large protein complexes

  102. Isolation and propagation of HIV-1 on peripheral blood mononuclear cells

  103. Isolation and short-term culture of primary keratinocytes, hair follicle populations and dermal cells from newborn mice and keratinocytes from adult mice for in vitro analysis and for grafting to immunodeficient mice

  104. Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue

  105. Isolation of DNA from small amounts of elephant ivory

  106. Isolation of intact RNA from cytometrically sorted Saccharomyces cerevisiae for the analysis of intrapopulation diversity of gene expression

  107. Isolation of mitochondria from rat brain using Percoll density gradient centrifugation

  108. L-Measure: a web-accessible tool for the analysis, comparison and search of digital reconstructions of neuronal morphologies

  109. Long PCR amplification of the entire mitochondrial genome from individual helminths for direct sequencing

  110. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products

  111. MRI of postmortem specimens of endangered species for comparative brain anatomy

  112. Measurement of chronic oxidative and inflammatory stress by quantification of isoketal/levuglandin ?-ketoaldehyde protein adducts using liquid chromatography tandem mass spectrometry

  113. Measuring elastase, proteinase 3 and cathepsin G activities at the surface of human neutrophils with fluorescence resonance energy transfer substrates

  114. Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data

  115. Measuring nanometer distances in nucleic acids using a sequence-independent nitroxide probe

  116. Membrane reconstitution of ABC transporters and assays of translocator function

  117. Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts

  118. Methods for detection, isolation and culture of mouse and human invariant NKT cells

  119. Methods for simultaneous measurement of apoptosis and cell surface phenotype of epithelial cells in effusions by flow cytometry

  120. Methods of using click chemistry in the discovery of enzyme inhibitors

  121. Methylation-sensitive single-nucleotide primer extension (Ms-SNuPE) for quantitative measurement of DNA methylation

  122. MicroRNA detection by northern blotting using locked nucleic acid probes

  123. MicroRNA expression profiling using microarrays

  124. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester

  125. Monitoring of cell therapy and assessment of cardiac function using magnetic resonance imaging in a mouse model of myocardial infarction

  126. Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing

  127. Mosher ester analysis for the determination of absolute configuration of stereogenic (chiral) carbinol carbons

  128. Multiparametric analysis of cells with different mitochondrial membrane potential during apoptosis by polychromatic flow cytometry

  129. Nanostructure analysis using spatially modulated illumination microscopy

  130. Neurologic assessment of somatosensory dysfunction following an experimental rodent model of cerebral ischemia

  131. Neutral red uptake assay for the estimation of cell viability/cytotoxicity

  132. Nitroreductase-mediated cell/tissue ablation in zebrafish: a spatially and temporally controlled ablation method with applications in developmental and regeneration studies

  133. Optimized mouse ES cell culture system by suspension growth in a fully defined medium

  134. Parallel tagged sequencing on the 454 platform

  135. Patent searches as a complement to literature searches in the life sciences?a 'how-to' tutorial

  136. Practical Proline-catalyzed asymmetric Mannich reaction of aldehydes with N-Boc-imines

  137. Practical guidelines for dual-color fluorescence cross-correlation spectroscopy

  138. Predicting the age of mosquitoes using transcriptional profiles

  139. Preparation and use of Leadfluor-1, a synthetic fluorophore for live-cell lead imaging

  140. Preparation and use of metal surface-immobilized DNA hairpins for the detection of oligonucleotides

  141. Preparation, application and testing of permanent antibacterial and antiviral coatings

  142. Preparation of chiral derivatives of ?-Ala containing the ?-phenylethyl group: useful starting materials for the asymmetric synthesis of ?-amino acids

  143. Preparation of iron oxide-based calcium sensors for MRI

  144. Preparation of large RNA oligonucleotides with complementary isotope-labeled segments for NMR structural studies

  145. Preparation of magnetic resonance contrast agents activated by ?-galactosidase

  146. Preparation of site-specifically labeled fluorinated proteins for 19F-NMR structural characterization

  147. Preparation of the functionalizable methionine surrogate azidohomoalanine via copper-catalyzed diazo transfer

  148. Preprocessing, classification modeling and feature selection using flow injection electrospray mass spectrometry metabolite fingerprint data

  149. Probe selection for DNA microarrays using OligoWiz

  150. Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research

  151. Production of a site- and phosphorylation state-specific antibody

  152. Production of neurons, astrocytes and oligodendrocytes from mammalian CNS stem cells

  153. Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips

  154. Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli

  155. Protocol for the induction of arthritis in C57BL/6 mice

  156. Protocol for the production of viable bimaternal mouse embryos

  157. Protocols for the in situ PCR-amplification and detection of mRNA and DNA sequences

  158. Purification and culture of nerve growth factor receptor (p75)-expressing basal forebrain cholinergic neurons

  159. Purification of matrix metalloproteinases by column chromatography

  160. Qualitative PCR?ELISA protocol for the detection and typing of viral genomes

  161. Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals

  162. Rapid solid-phase synthesis of a calmodulin-binding peptide using controlled microwave irradiation

  163. Reduction of dynamic protein concentration range of biological extracts for the discovery of low-abundance proteins by means of hexapeptide ligand library

  164. Reprogramming the amino-acid substrate specificity of orthogonal aminoacyl-tRNA synthetases to expand the genetic code of eukaryotic cells

  165. Retrovirally mediated telomerase immortalization of neural progenitor cells

  166. Rice blast infection of Brachypodium distachyon as a model system to study dynamic host/pathogen interactions

  167. Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae

  168. Silver staining DNA in polyacrylamide gels

  169. Simple protocols for NMR analysis of the enantiomeric purity of chiral diols

  170. Simple protocols for NMR analysis of the enantiomeric purity of chiral primary amines

  171. Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy

  172. Single-channel recording of ligand-gated ion channels

  173. Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins

  174. Site-specific incorporation of nitroxide spin-labels into 2?-positions of nucleic acids

  175. Solid-phase synthesis of pseudo-complementary peptide nucleic acids

  176. Southwestern blotting in investigating transcriptional regulation

  177. Spatially and temporally controlled electroporation of early chick embryos

  178. Study and selection of in vivo protein interactions by coupling bimolecular fluorescence complementation and flow cytometry

  179. Substrate-induced gene expression (SIGEX) screening of metagenome libraries

  180. Synthesis, encapsulation, purification and coupling of single quantum dots in phospholipid micelles for their use in cellular and in vivo imaging

  181. Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase

  182. Synthesis of lactones using substituted benzoic anhydride as a coupling reagent

  183. Synthesis of poly(L-lactide) and polyglycolide by ring-opening polymerization

  184. Synthesis of pyrimidines by direct condensation of amides and nitriles

  185. Synthesis of the functionalizable methionine surrogate azidohomoalanine using Boc-homoserine as precursor

  186. Tetrazolium-based colorimetric assay for the detection of HIV replication inhibitors: revisited 20 years later

  187. The 32P-postlabeling assay for DNA adducts

  188. The application of the 5-choice serial reaction time task for the assessment of visual attentional processes and impulse control in rats

  189. The detection and quantification of growth cone collapsing activities

  190. The mouse cornea micropocket angiogenesis assay

  191. The partial reduction of electron-deficient pyrroles: procedures describing both Birch (Li/NH3) and ammonia-free (Li/DBB) conditions

  192. The prion organotypic slice culture assay?POSCA

  193. The selective detection of mitochondrial superoxide by live cell imaging

  194. The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability

  195. Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression

  196. Tracking intracellular protein movements using photoswitchable fluorescent proteins PS-CFP2 and Dendra2

  197. Transient expression of fluorescent fusion proteins in protoplasts of suspension cultured cells

  198. Two-dimensional gel electrophoresis for identifying proteins that bind DNA or RNA

  199. Use of Raman spectroscopy as a tool for in situ monitoring of microwave-promoted reactions

  200. Use of high-performance anion exchange chromatography with pulsed amperometric detection for O-glycan determination in yeast

  201. Using the ?-lactamase protein-fragment complementation assay to probe dynamic protein?protein interactions

  202. Version 1.2 of the Crystallography and NMR system

  203. Visualization of protein interactions in living Caenorhabditis elegans using bimolecular fluorescence complementation analysis

    204. Find this article in Google