An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in Arabidopsis plants
Chromatin immunoprecipitation (ChIP) is a powerful tool for the characterization of covalent histone modifications and DNA?histone interactions in vivo. The procedure includes DNA?histone cross-linking in chromatin, shearing DNA into smaller fragments, immunoprecipitation with antibodies against the histone modifications of interest, followed by PCR identification
The historic takeover of Fannie Mae and Freddie Mac, which could come as soon as this weekend, moved to the forefront of the presidential campaign Saturday.
In the arena that night, the whole last minute was drowned out by cheers — and then when the soaring music swelled, the confetti rained down as the harbinger of balloons and the hopeful first family took the stage, forget about it — it was a perfect end to a convention that last Monday, no one even knew if it would happen. But it did, and I'm so glad to have been there.
