Suspended-drop electroporation for high-throughput delivery of biomolecules into cells
We present a high-throughput method that enables efficient delivery of biomolecules into cells. The device consists of an array of 96 suspended electrode pairs, where small sample volumes are top-loaded, electroporated and bottom-ejected into 96-well plates. We demonstrate the use of this suspended-drop electroporation (SDE) device to effectively introduce fluorescent dextran, small interfering RNA (siRNA) or cDNA into primary neurons, differentiated neutrophils and other cell types with conventionally low transfection rates.
AP - The plan was nothing if not audacious: A turncoat persuades rebels to bring together their most prized hostages and march them 90 miles through Colombia's wilderness. A month later, disguised commandos primed with acting lessons land in a helicopter and trick the rebels into handing them over.
Reuters - The investigation of a salmonella
outbreak in the United States is shifting to the southern
border to encompass produce imported from Mexico, CNN reported
on Thursday.
