Isoform discovery by targeted cloning, 'deep-well' pooling and parallel sequencing
The complete set of coding sequences, including all splice isoforms, is not known for any metazoan organism. Combination of a normalized pooling scheme and a new assembly algorithm with 454 sequencing yields a methodological pipeline for isoform discovery. The validated pipeline may now be applied genome-wide.
In a sharp turnaround, the Republican candidates has opened a 5-point lead on his Democratic rival and is seen as a stronger manager of the economy, according to a Reuters/Zogby poll.
AFP - Georgia's South Ossetia and Abkhazia regions planned rallies Thursday to ask Russia for recognition as independent states, two weeks after Russian troops occupied both provinces.
