A probability-based approach for the analysis of large-scale RNAi screens
We describe a statistical analysis methodology designed to minimize the impact of off-target activities upon large-scale RNA interference (RNAi) screens in mammalian cells. Application of this approach enhances reconfirmation rates and facilitates the experimental validation of new gene activities through the probability-based identification of multiple distinct and active small interfering RNAs (siRNAs) targeting the same gene. We further extend this approach to establish that the optimal redundancy for efficacious RNAi collections is between 4?6 siRNAs per gene.
Senate Democrats hinted at payback for Sen. Joe Lieberman, who called Barack Obama an untested candidate beholden to Democratic interest groups in a prime-time GOP convention address.
AFP - Authorities in China's Muslim-populated far northwest are seeking to prevent mass prayers and the distribution of religious material as part of a security crackdown for Ramadan, government notices said.
Reuters - Condoleezza Rice travels to Libya on
Friday, the first trip there by a U.S. secretary of state in 55
years, signaling improved ties between the two countries.
