CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammatory response, whereas CpG-free pDNA vectors do not. Using a CpG-free pDNA expression vector, we achieved sustained (?56 d) in vivo transgene expression in the absence of lung inflammation.
The Republican presidential candidate is seeking to bolster his appeal to voters on economic issues by speaking to some of those hardest hit in the recent downturn: auto workers.
AFP - Pope Benedict XVI on Sunday urged hundreds of thousands of young Catholics to beat back a "spiritual desert" spreading through the modern world as he closed Catholic World Youth Day in Australia.
Reuters - Tens of thousands of people filled
Nicaragua's capital on Saturday to celebrate the country's 1979
leftist revolution, giving a lift to President Daniel Ortega as
his government faces simmering protests.
